Impairment of renal blood flow after renal ischaemia–reperfusion is not aggravated by low plasma levels of arginine. BJS 2000; 87: 935-935.
Published: 6th December 2002
Authors: R. J. Nijveldt, H. A. Prins, J. A. Rauwerda, A. A. van Lambalgen, T. Teerlink, P. A. M. van Leeuwen et al.
The fundamental role of the
In this unilateral renal ischaemia–reperfusion model, male Wistar rats were used. Arginase infusion (ASE) was used to lower arginine plasma levels to 50 per cent of normal; control rats received saline infusion (SAL). After an ischaemic period of 90 min, the kidney was perfused for 150 min (IR kidney). The contralateral kidney was left in situ and served as a control (CL kidney). Blood flow measurement was performed at the end of the experiment using radiolabelled microspheres. Blood samples were taken for amino acid analysis (high‐pressure liquid chromatography).
Infusion of arginase efficiently decreased plasma levels of arginine (ASE mean(s.d.) 45·3(4·8) μmol/dm3 versus SAL 107·5(6·0) μmol/dm3; P < 0·0001). In both SAL and ASE rats, a significantly lower blood flow was found in the IR kidney compared with the (non‐ischaemic) CL kidney. Lowering arginine plasma levels by arginase did not influence renal blood flow compared with SAL, in both IR and CL kidneys.
In this acute, unilateral renal ischaemia–reperfusion model, low arginine plasma levels did not further aggravate renal blood flow. © 2000 British Journal of Surgery Society LtdFull text