P‐selectin mediates neutrophil rolling and recruitment in acute pancreatitis. BJS 2012; 99: 246-255.
Published: 23rd November 2011
Authors: H. Hartman, A. Abdulla, D. Awla, B. Lindkvist, B. Jeppsson, H. Thorlacius et al.
The adhesive mechanisms regulating leucocyte–endothelium interactions in the pancreas remain elusive, but selectins may play a role. This study examined the molecular mechanisms mediating leucocyte rolling along the endothelium in the pancreas and the therapeutic potential of targeting the rolling adhesive interaction in acute pancreatitis (AP).
Pancreatitis was induced by retrograde infusion of 5 per cent sodium taurocholate into the pancreatic duct, repeated intraperitoneal administration of caerulein (50 µg/kg) or intraperitoneal administration of
Taurocholate infusion into the pancreatic duct increased the serum level of trypsinogen, trypsinogen activation, pancreatic neutrophil infiltration, macrophage inflammatory protein (MIP) 2 formation and tissue damage. Immunoneutralization of P‐selectin decreased the taurocholate‐induced increase in serum trypsinogen (median (range) 17·35 (12·20–30·00) versus 1·55 (0·60–15·70) µg/l; P = 0·017), neutrophil accumulation (4·00 (0·75–4·00) versus 0·63 (0–3·25); P = 0·002) and tissue damage, but had no effect on MIP‐2 production (14·08 (1·68–33·38) versus 3·70 (0·55–51·80) pg/mg; P = 0·195) or serum trypsinogen activating peptide level (1·10 (0·60–1·60) versus 0·45 (0–1·80) µg/l; P = 0·069). Intravital fluorescence microscopy revealed that anti‐P‐selectin antibody inhibited leucocyte rolling completely in postcapillary venules of the inflamed pancreas.
Inhibition of P‐selectin protected against pancreatic tissue injury in experimental pancreatitis. Targeting P‐selectin may be an effective strategy to ameliorate inflammation in AP. Copyright © 2011 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.Full text